Master student - Life Sciences, Biology (m/f/d)

The project: CRISPR/Cas9 has revolutionized genome engineering, transforming both basic research and translational medicine. As we continue to expand the potential of this groundbreaking technology, it is crucial to address its limitations. One key challenge is the lack of perfect precision and predictability in genome editing outcomes. CRISPR/Cas9 induces double-strand breaks (DSBs) in DNA, which are often repaired by error-prone mechanisms. These repair processes can lead to unintended large genomic alterations at the on-target site, potentially resulting in harmful effects. The extent and frequency of such events, however, remain poorly understood. It is still unclear whether these large deletions are influenced by specific cell types or by the genome editing reagents used. This project will also explore prime editing, a next-generation CRISPR-based technique, to evaluate whether it offers improved precision and reduced risk of large deletions compared to CRISPR/Cas9.

The aim of this Master's thesis is to establish a robust and comprehensive assessment of potential on-target effects arising from different genome editing mechanisms. To achieve this, a CRISPR-based screen will be performed in both induced pluripotent stem cells (iPSCs) and established cell lines. Given the pivotal role of iPSCs in translational medicine, it is essential to evaluate the risk of unintended editing outcomes that could compromise their therapeutic potential. The project involves meticulous experimental design and the application of state-of-the-art genome editing technologies, including high-throughput next-generation sequencing (NGS) and digital PCR.

We are looking for a highly motivated Master's student (f/m/d) with strong commitment, curiosity, and team spirit. Applicants should hold a completed Bachelor's degree in Life Sciences, Biology, or a related field. If you're passionate about advancing the frontiers of genome engineering, applying innovative techniques, and tackling critical questions in gene editing, we would love to hear from you. As part of our group, you will have the opportunity to contribute to pioneering research aimed at uncovering the complexities of CRISPR/Cas9 and prime editing technologies, while helping to develop strategies to mitigate associated risks.

Join an international and collaborative research environment within the Environmental Adaptation and Cellular Resilience group, led by Dr. Andrea Rossi. You’ll work closely with leading scientists and gain hands-on experience with cutting-edge tools and platforms, including multiple types of high-throughput sequencing technologies, digital PCR, and advanced genome engineering techniques. You’ll be fully integrated into a dynamic and supportive team, have access to state-of-the-art facilities, and contribute to impactful research with the goal of publishing your results in a peer-reviewed scientific journal. This is a unique opportunity to be at the forefront of biomedical innovation and make a meaningful contribution to the future of biotechnology and regenerative medicine.

Start: As soon as possible